LOCUS       XYLT1-pGEn2             8070 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_recomb     complement(3839..3867)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    6698..7239
                     /note="WPRE"
     misc_recomb     6660..6682
                     /label=attB2
                     /note="attB2 site"
                     /note="recognized as attB2"
     misc_feature    complement(7246..7460)
                     /note="BGH pA"
     misc_feature    complement(7548..7677)
                     /note="3'-ITR"
     misc_feature    2982..3056
                     /note="TCM signal sequence"
     misc_feature    3060..3083
                     /note="His tag"
     misc_feature    3084..3134
                     /note="AviTag"
     misc_feature    3135..3830
                     /note="Superfolder GFP"
     misc_feature    2976..2981
                     /note="Kozak seq"
     misc_feature    3868..3890
                     /note="TEV site"
     misc_feature    3891..6653
                     /note="OPTIMIZED XYLT1 coding region"
     vector          join(6668..8070,1..3857)
                     /source="pGEn2-DEST"
                     /type="Destination Vector"
     clone           3858..6667
                     /source="XYLT1 pDONR"
                     /type="Entry Clone"
     source          1..8070
                     /dnas_title="XYLT1-pGEn2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC
     3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC
     3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA
     3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG
     3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG
     3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC
     3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG
     3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC
     3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG
     3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA
     3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA
     3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG
     3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
     3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG
     3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT
     3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc GATAGCGGAG
     3901 CCGGCGAGAG AAGAGGCGGA GCTGCTGTGG GAGGGGGAGA ACAGCCTCCT CCAGCTCCTG
     3961 CTCCTAGAAG AGAGAGAAGG GATCTGCCTG CCGAACCTGC TGCTGCTAGA GGTGGTGGTG
     4021 GCGGAGGCGG GGGTGGCGGG GGAGGAAGAG GACCTCAGGC TAGAGCTAGA GGCGGCGGAC
     4081 CTGGCGAACC TAGAGGACAG CAGCCAGCTA GTAGAGGCGC TCTGCCAGCA AGAGCACTGG
     4141 ATCCTCACCC TAGCCCTCTG ATCACCCTGG AAACCCAGGA TGGCTACTTC AGCCACCGGC
     4201 CCAAAGAAAA AGTGCGGACC GACAGCAACA ACGAGAACTC CGTGCCCAAG GACTTCGAGA
     4261 ACGTGGACAA CAGCAACTTC GCCCCCAGAA CACAGAAGCA GAAGCACCAG CCTGAGCTGG
     4321 CCAAGAAGCC CCCTAGCAGA CAGAAAGAAC TGCTGAAGCG GAAGCTGGAA CAGCAGGAAA
     4381 AGGGCAAGGG CCACACCTTT CCAGGCAAAG GACCTGGGGA AGTGCTGCCT CCTGGCGATA
     4441 GAGCCGCCGC TAATTCTTCT CACGGCAAGG ACGTGTCCAG ACCCCCTCAT GCTAGAAAGA
     4501 CAGGCGGCAG CAGCCCCGAG ACTAAGTACG ATCAGCCTCC CAAGTGCGAC ATCAGCGGCA
     4561 AAGAAGCCAT CAGCGCCCTG AGCAGAGCCA AGTCCAAGCA CTGTAGACAG GAAATCGGCG
     4621 AGACATACTG CCGGCACAAG CTGGGACTGC TGATGCCCGA GAAAGTGACC AGATTCTGCC
     4681 CTCTGGAAGG CAAGGCCAAC AAGAACGTGC AGTGGGACGA GGATAGCGTC GAGTACATGC
     4741 CTGCCAATCC TGTGCGGATC GCCTTTGTGC TGGTGGTGCA TGGAAGGGCT TCCCGGCAGC
     4801 TGCAGAGAAT GTTCAAGGCC ATCTACCACA AGGACCACTT CTACTACATC CATGTGGACA
     4861 AGCGGAGCAA CTACCTGCAC AGACAGGTCC TCCAGGTGTC CAGGCAGTAT AGCAATGTGC
     4921 GCGTGACCCC TTGGAGAATG GCCACAATTT GGGGAGGCGC TAGCCTGCTG AGCACATACC
     4981 TGCAGAGCAT GCGGGACCTG CTGGAAATGA CCGACTGGCC CTGGGACTTC TTCATCAACC
     5041 TGAGCGCCGC TGACTACCCC ATCAGGACCA ATGATCAGCT GGTGGCCTTC CTGAGCCGGT
     5101 ACAGAGATAT GAACTTCCTG AAAAGCCACG GCCGGGACAA CGCCCGGTTT ATCAGAAAGC
     5161 AGGGCCTGGA CCGGCTGTTC CTGGAATGTG ATGCTCATAT GTGGCGGCTG GGCGACAGAA
     5221 GAATCCCTGA GGGAATTGCC GTGGATGGCG GCTCCGACTG GTTTCTGCTG AATAGAAGAT
     5281 TCGTGGAATA CGTGACCTTC AGCACCGACG ACCTGGTCAC CAAGATGAAG CAGTTTTACA
     5341 GCTACACCCT GCTGCCCGCC GAGAGCTTTT TTCACACCGT GCTCGAGAAT AGCCCCCACT
     5401 GCGATACCAT GGTGGATAAC AACCTGCGGA TCACCAACTG GAACCGGAAA CTGGGCTGCA
     5461 AGTGCCAGTA CAAGCACATC GTGGATTGGT GCGGCTGCTC CCCCAATGAC TTCAAGCCAC
     5521 AGGACTTCCA CAGATTCCAG CAGACCGCCA GACCCACCTT CTTCGCCAGA AAGTTCGAGG
     5581 CCGTGGTCAA CCAGGAAATT ATCGGCCAGC TGGACTACTA CCTGTACGGA AACTACCCTG
     5641 CCGGAACCCC TGGCCTGAGA AGCTACTGGG AGAATGTGTA CGACGAGCCC GATGGCATCC
     5701 ACAGCCTGTC TGATGTGACC CTGACCCTGT ACCACAGCTT TGCCAGACTG GGGCTGAGAA
     5761 GGGCCGAGAC ATCTCTGCAC ACCGACGGCG AAAACAGCTG TAGGTACTAC CCTATGGGCC
     5821 ACCCTGCCAG CGTGCACCTC TATTTTCTGG CCGACCGGTT TCAGGGTTTC CTGATCAAGC
     5881 ACCACGCCAC CAATCTGGCC GTGTCCAAGC TCGAGACACT GGAAACATGG GTCATGCCCA
     5941 AAAAGGTGTT CAAGATCGCC AGCCCCCCCA GCGATTTTGG CAGACTGCAG TTTAGCGAAG
     6001 TGGGCACCGA TTGGGACGCC AAAGAGAGAC TGTTCAGAAA CTTTGGAGGC CTGCTGGGCC
     6061 CCATGGATGA ACCTGTGGGA ATGCAGAAGT GGGGAAAGGG CCCCAATGTG ACAGTGACCG
     6121 TGATTTGGGT GGACCCCGTG AACGTGATCG CCGCCACCTA CGATATCCTG ATCGAGAGCA
     6181 CAGCCGAGTT CACCCACTAC AAGCCTCCAC TGAATCTGCC TCTCAGACCC GGCGTGTGGA
     6241 CAGTGAAAAT TCTGCACCAT TGGGTGCCCG TGGCCGAAAC AAAATTCCTG GTGGCTCCCC
     6301 TGACCTTCTC CAACAGACAG CCCATTAAGC CCGAAGAGGC CCTGAAGCTG CACAACGGCC
     6361 CACTGAGAAA CGCCTACATG GAACAGAGCT TCCAGTCCCT GAATCCCGTG CTGAGCCTGC
     6421 CTATCAATCC TGCCCAGGTG GAACAGGCCA GAAGAAATGC CGCTTCTACC GGCACTGCAC
     6481 TCGAGGGATG GCTGGATTCT CTCGTGGGCG GAATGTGGAC CGCCATGGAT ATTTGTGCCA
     6541 CCGGACCTAC CGCTTGCCCT GTGATGCAGA CATGTAGCCA GACCGCTTGG AGCAGCTTTA
     6601 GCCCCGATCC TAAGTCTGAA CTGGGCGCCG TGAAACCTGA CGGCAGACTC AGATAGGACC
     6661 CAGCTTTCTT GTACAAAGTG GTTGATAGCT TGGATCCAAT CAACCTCTGG ATTACAAAAT
     6721 TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC
     6781 TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT
     6841 GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG
     6901 CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG
     6961 TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC
     7021 CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT
     7081 GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT
     7141 GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG
     7201 CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGA GATCTGCCTC GACTGTGCCT
     7261 TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT
     7321 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG
     7381 TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC
     7441 AATAGCAGGC ATGCTGGGGA CTCGAGTTAA GGGCGAATTG CCGATTAGGA TCTTCCTAGA
     7501 GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT
     7561 GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA
     7621 GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT
     7681 TAATTAAGAG CTCATGGCGC GCCTAGGCCT TGACGGCCTT CCGCCAATTC GCCCTATAGT
     7741 GAGTCGTATT ACGTCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC
     7801 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG
     7861 CGAAGAGGCC CGCACCGAAA CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG
     7921 AGCGCCCTGT AGCGGCCACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT
     7981 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT
     8041 TTTAACAAAA TATTAACGCT TACAATTTAG
//

